![]() Place the bottle containing the mixture into a pressure cooker or autoclave.Put tin foil around the cap and neck of the bottle.Put the lid on and place tin foil over the top. Measure out 400ml of water (preferably distilled OR grain water) and pour this through the funnel also.Weigh out 6g BRF, 9g Agar into any bowl of your choosing and pour the mixture into your bottle using a food funnel.Pressure Cooker (A post on my recommendations).Alongside this, there is reduced condensation inside the finished product. It is a lot easier to sterilize one bottle of the mixture then pour it out and inoculate in one sitting. The reason people usually use this technique instead of the previous is due to speed and efficiency. This is due to the high likelihood of airborne contaminants getting onto your plates if you pour the mixture in the open air. Option 2 – Inoculate through the hole in the top of the container using a syringe (created before sterilization) then cover with micro-pore tape.įor poured Agar tek, a “ still air box or laminar flow hood” is definitely required. Option 1 – Place all your containers inside a still airbox and then inoculate them with spores/spawn/culture one by one whilst following the sterile technique. Allow the pressure cooker to cool overnight.Sterilize the contents on 15psi for a minimum of 30minutes.Place the containers into your pressure cooker and fill the pressure cooker with two cups of water.Place foil on the lid of each container OR stack the containers and wrap them in foil.Wipe away any excess condensation around the outside of your containers.Leave the mixture to cool until the liquid sets into a solid jelly-like substance.Transfer the mixture into containers that can be used inside a pressure cooker at 15psi (Petri dish, ketchup holders, etc).Heat the mixture until it begins to boil, continue stirring until the mixture begins to froth and rise.Measure out 400ml of water (preferably distilled OR grain water) and pour this over the mixture then stir.Weigh out 6g BRF, 9g Agar into any bowl of your choosing and pour the mixture into a saucepan.Containers or Polypropylene petri dishes (usable in a pressure cooker).Pressure Cooker ( pressure cookers for mycology).If your sterile technique is good you will likely avoid contamination, this is how I first started using agar. We recommend the use of a “still air box” (I have a post here on how to make one) for the non pour BRF recipe however there are methods that can be applied to get around the need for one.įor example, it is possible to put a small hole in the lid of each container then cover it with micropore tape, when it comes to inoculation you can inject through this hole, then cover it back up. As a result, the nutritious medium is less exposed to airborne contaminants. This guide is the easier guide of the two due to the fact that no pouring is required.
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